首页 > 分享 > 特应性皮炎模型小鼠的生长繁殖及血液生理生化指标 growth, reproduction and blood physiological and biochemical indexes in an atopic dermatitis mouse model

特应性皮炎模型小鼠的生长繁殖及血液生理生化指标 growth, reproduction and blood physiological and biochemical indexes in an atopic dermatitis mouse model

萌宠菠菠乐园
2024-08-10 06:38

中国组织工程研究第17卷第41期2013–10–08出版ChineseJournalofTissueEngineeringResearchOctober8,2013Vol.17,No.41doi:10.3969/j.issn.2095-4344.2013.41.015[http://www.crter.org]童敏梁云生张桂英.特应性皮炎模型小鼠的生长繁殖及血液生理生化指标[J].中国组织工程研究201317(41):7284-7289.P.O.Box1200,Shenyang110004www.CRTER.org

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www.CRTER.org童敏★女1971年生湖南省长沙市人汉族2008年中南大学毕业硕士副主任技师主要从事实验动物药理学研究。tongmin22626@163.com通讯作者**英教授硕士生导师**大学湘雅二医院皮肤性病科湖南省长沙市410011zhangguiying67@yahoo.com.cn中图分类号:R318文献标识码:B文章编号:2095-4344(2013)41-07284-06收稿日期2013-06-25修回日期2013-07-12(201305076/G·C)TongMin★,Master,Associatechieftechnician,ExperimentalAnimalCenter,theSecondXiangyaHospitalofCentralSouthUniversity,Changsha410000,HunanProvince,Chinatongmin22626@163.comCorrespondingauthor:ZhangGui-ying,Professor,Master’ssupervisor,DepartmentofDermatologyandVenereology,theSecondXiangyaHospitalofCentralSouthUniversity,Changsha410000,HunanProvince,Chinazhangguiying67@yahoo.com.cnReceived:2013-06-25Accepted:2013-07-12特应性皮炎模型小鼠的生长繁殖及血液生理生化指标*★童敏1梁云生2张桂英2(中南大学湘雅二医院1实验动物中心2皮肤性病科湖南省长沙市410011)文章亮点1日本NC/Nga鼠可自发产生特应性皮炎样皮损其皮损、病理和免疫学特征等均与人类特应性皮炎相一致作为特应性皮炎动物模型的应用价值日益显现。目前NC/Nga鼠在国内还没有相关单位研究饲养繁殖对NC/Nga鼠进行深入研究可望自主开发出特应性皮炎动物模型。2实验检测了Nc/Nga小鼠的生长繁殖数据和部分血液生理生化指标结果提示性别和年龄都会影响Nc/Nga小鼠的血液生理和生化值指标。3实验结果为科研人员使用Nc/Nga小鼠从事特应性皮炎医药研究提供了基础对照参考数据建立了Nc/Nga小鼠生长繁殖及血液生化等指标的数据档案同时亦为该小鼠标准化鉴定和质量控制提供了依据。关键词组织构建组织构建实验造模Nc/Nga鼠特应性皮炎生长率血液生理生化指标分娩间隔产仔数离乳率省级基金主题词皮肤疾病模型动物生长繁殖血液化学分析基金资助湖南省科研条件创新专项计划资助项目(2011TT2019)*摘要背景Nc/Nga小鼠皮损、病理和免疫学特征等均与人类特应性皮炎相一致作为特应性皮炎动物模型其研究价值日益显现。目前国内尚无Nc/Nga小鼠的生理生化指标等相关数据报道。目的观察特应性皮炎动物模型Nc/Nga小鼠的生长繁殖性能以及血液生理、生化指标。方法统计Nc/Nga小鼠1-3胎的繁殖指标数据包括每胎产仔数、离乳率、怀孕率及胎间隔。分别称取60只1-56dNc/Nga仔鼠的体质量雌雄各半绘制其生长表格。Nc/Nga鼠眼眶采血测定其血常规和生化值。结果与结论Nc/Nga小鼠平均交配分娩间隔为(25.8±3.1)d平均产仔数为(7.5±2.5)只平均离乳率为(97.2±1.2)%、平均怀孕率为(97.0±1.4)%1-3胎间差异无显著性意义(P>0.05)。Nc/Nga小鼠的体质量随着日龄的增长而逐渐增加断奶后一两周内增重最为迅速6周后体质量雌雄间差异无显著性意义(P>0.05)。同一日龄的雌雄间血常规和生化值比较雌性小鼠红细胞、血红蛋白明显高于雄性小鼠雄性小鼠血小板计数明显高于雌性小鼠(P<0.05)雌性小鼠三酰甘油、白蛋白水平明显高于雄性小鼠(P<0.05)。提示性别和年龄都会影响Nc/Nga小鼠的血液生理和生化值指标。Growth,reproductionandbloodphysiologicalandbiochemicalindexesinanatopicdermatitismousemodelTongMin1,LiangYun-sheng2,ZhangGui-ying2(1ExperimentalAnimalCenter,2DepartmentofDermatologyandVenereology,theSecondXiangyaHospitalofCentralSouthUniversity,Changsha410000,HunanProvince,China)AbstractBACKGROUND:Theskinlesions,pathologicalandimmunologicalcharacteristicsofNc/Ngamiceareconsistentwithhumanatopicdermatitis,andasanatopicdermatitisanimalmodel,ithasgreatresearchvalue.ButtherearenoreportsonthephysiologicalandbiochemicalparametersofNc/NgamiceinChina.OBJECTIVE:ToobservethegrowthandreproductionandbloodphysiologicalandbiochemicalparametersofatopicdermatitismodelofNC/NgamiceMETHODS:ThedataofreproductiveperformanceofNC/Ngamicefromthefirsttothethirdgenerationwasanalyzed,includingmeanlittersize,weaningrate,pregnancyrateandgenerationinterval.Thebodymassof60Nc/Ngamicewith1-56daysoldwasmeasured,30miceoffemaleandmale,thenthegrowthcurvewasdraw.Thebloodsamplesfromtheinfraorbitalvesselswerecollectedtodetectthebloodphysiologicalandbiochemicalparametersinthemice.RESULTSANDCONCLUSION:ThemeandeliveryintervalofNc/Ngamicewas(25.8±3.1)days,meanlittersizewas(7.5±2.5)mice,meanweaningratewas(97.2±1.2)%,themeanpregnancyratewas(97.0±1.4)%,andtherewasnosignificantdifferenceamongthethreegenerationsofthesemice(P>0.05).ThebodymassofNc/Nga