动物骨关节炎模型病理评分OARSI标准
“骨关节炎是常用的动物模型，标本取材后该做什么分析？来看看病理切片的OARSI关节炎软骨损伤标准进行评分。给您的论文图片加点干货把”
首先需要说明的是，OARSI是基于关节软骨病理切片分析评分的方法。待分析标本在常规包埋后，进行冠状位切片，染色后在镜下观察并评分。主要包括两个方面：关节软骨损伤及骨赘生成情况。
观察和评分区域需要包含以下四部分的关节面（关节软骨）：股骨内侧髁、股骨外侧髁、胫骨内侧平台和胫骨外侧平台。对四个部分分别进行评分求和得到最终评分。
0.5=非常小的退变。蓝色（或其他阳离子染料）染色丢失小部分（蛋白多糖损失），但是没有结构变化。
1=很小的退变：可见小的表面磨损及软骨表面下的纤维化，没有软骨细胞或软骨基质的损失，损伤面积小于总面积的5%。
2=轻度退变：损伤垂直及软骨表层下方，但很少延伸到更深的软骨层。软骨表面基质有部分损失，或软骨细胞/蛋白多糖可见局部区域损失，但是仍有良好的胶原蛋白保存，软骨损伤面积占总软骨表面的5%到10%左右。
3=中度退变：损伤垂直向下累及到钙化软骨层，软骨细胞/蛋白多糖的局部区域丢失，约10%至24%的关节软骨受累及。
4=明显退变：垂直裂隙/损伤延伸到钙化软骨下，超过关节表面的25% 至 50%的软骨，或软骨细胞/蛋白多糖的局部区域丢失，累及关节软骨厚度的25% 至 50%。
5=严重退变：垂直裂隙/破坏延伸到关节钙化软骨下方，50%到75%的软骨表面受损，或软骨细胞/蛋白多糖的局部区域丢失，约50%到75%软骨厚度受损。
6=非常严重的退化：软骨的垂直裂隙/破坏延伸到钙化软骨下方，大于75%的关节表面受损，可能仅剩少量无细胞胶原蛋白区域。
注意：如果切面不在承重区域内，则不给出分数并记录 NA。关节软骨退化总和=计算所有四个表面的软骨退化分数的总和，最大值为24。
最大的骨赘（胫骨或股骨）使用目镜测量。根据测量大小进行如下打分：
关节评价总分=通过将内侧和外侧骨赘评分与总关节软骨退化总和相加来计算总关节评分。最大值为 30。
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Grading of Osteoarthritic Cartilage Damage (OARSI Criteria)
0.5 = Very Minimal Degeneration: Loss of t. blue (or other cationic dye) stain (proteoglycan loss) without structural changes
1 = Minimal Degeneration: Small surface to subsurface fibrillations without major loss of chondrocytes or cartilage matrix, may have small focal area of chondrocyte loss extending partial thickness over less than 5% of total surface
2 = Mild Degeneration: Vertical clefts down to the layer immediately below the superficial layer with few extending deeper and some loss of surface matrix, or focal areas of chondrocyte/proteoglycan loss with good collagen preservation extending partial thickness over 5 to 10% of the surface
3 = Moderate Degeneration: Vertical clefts/erosion to the calcified cartilage extending over <25% of the articular surface, or focal areas of chondrocyte/proteoglycan loss with some collagen preservation extending full thickness over 10 to 24% of the surface
4 = Marked Degeneration: Vertical clefts/erosion to the calcified cartilage extending over 25 to 50% of the articular surface, or focal areas of chondrocyte/proteoglycan loss with some collagen preservation extending full thickness over 25 to 50% of the surface
5 = Severe Degeneration: Vertical clefts/erosion to the calcified cartilage extending over 50 to 75% of the articular surface, or focal areas of chondrocyte/proteoglycan loss with some collagen preservation extending full thickness over 50 to 75% of the surface
6 = Very Severe Degeneration: Vertical clefts/erosion to the calcified cartilage extending >75% of the articular surface, may be few areas of acellular collagen remaining
If a surface is not in the section or not in the load bearing area then no score is given and NA is recorded.
Total Joint Cartilage Degeneration Sum
The sum of the cartilage degeneration scores for all four surfaces is calculated. The maximum value is 24.
Osteophyte Score and Measurement
The largest osteophyte (tibia or femur) is measured using an ocular micrometer. A score is assigned based on the measurement as follows:
2 = Medium (151 to 300 µm)
The total joint score is calculated by adding the medial and lateral osteophyte scores to the total joint cartilage degeneration sum. The maximum value i
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