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巴马长寿饮食模式在衰老小鼠模型中的抗氧化应激效果

萌宠菠菠乐园
2024-10-31 21:14

摘要： 为了验证巴马长寿饮食模式的抗衰老作用，通过给小鼠皮下注射D-半乳糖（500 mg/（kg·d））制备衰老模型，根据本团队前期研究结果，选取巴马长寿老人饮食中具有代表性的营养特征（能量限制及VA、大豆异黄酮、膳食纤维、微量元素铁、锰、钴、硒的摄入），分别设计了能限组、胡豆组、纤维组、元素组和复合组饮食，并以自由采食的方式饲喂小鼠，连续8 周。对小鼠血清、肝脏和脑组织中丙二醛（malondialdehyde，MDA）水平、血清和脑组织中总超氧化物歧化酶（total superoxide dismutase，T-SOD）活力、肝脏和脑组织中总抗氧化能力（total antioxidant capacity，T-AOC）进行测定。结果表明：与衰老组小鼠相比，5 种特征饮食都能抑制小鼠体内MDA的蓄积，提高抗氧化酶活力和T-AOC；通过正向化和无量纲化处理，发现复合组饮食抗氧化应激效果最强，能显著抑制衰老小鼠血清、肝脏和脑组织中MDA蓄积（P＜0.05），使血清中T-SOD活力增强12.33%（P＜0.05），并使肝脏中T-AOC显著提高35.35%（P＜0.05）。5 种模式综合量化评价的顺序为：复合组＞胡豆组＞能限组＞纤维组＞元素组。说明巴马长寿饮食模式对D-半乳糖致衰老小鼠有明显的抗氧化应激作用，这也预示着其具有较显著的抗衰老作用效果和良好的开发潜力。

关键词: 巴马长寿饮食模式, 抗氧化应激, D-半乳糖, 衰老模型

Abstract: In order to validate the anti-aging effect of Bama longevity dietary patterns, a mouse model of aging was established by subcutaneously injecting D-galactose (500 mg/(kg·d)) into animals once daily for eight consecutive weeks. Meanwhile, the representative nutrients in Bama longevity dietary patterns (including calorie restriction and intake of VA and soybean isoflavones, dietary fiber and trace elements such as iron, manganese, cobalt and selenium) were selected to design five characteristic diets, calorie restriction, VA and soybean isoflavones, dietary fiber, trace elements and compound diet, for feeding aging mice. The contents of malondialdehyde (MDA) in serum, liver and brain, the activity of total superoxide dismutase (T-SOD) in serum and brain, and total antioxidant capacity (T-AOC) in liver and brain were measured. The results showed that all five diets could inhibit the accumulation of MDA in aging mice, while the activity of antioxidant enzymes and T-AOC were improved when compared with the aging model group. Through positive processing and nondimensionalization, it was found that compound nutrition had the best anti-oxidative stress effect , which could inhibit the accumulation of MDA in serum, liver and brain (P < 0.05), increase the activity of T-SOD by 12.33% (P < 0.05), and significantly increase the activity of T-AOC in liver by 35.35% (P < 0.05). A comprehensive evaluation showed that the antiaging effect of 5 diets followed the decreasing order of compound diet > VA and soybean isoflavones > calorie restriction > dietary fiber > trace elements. These results demonstrate that the Bama longevity diet pattern has a significant protective effect against oxidative stress in D-galactose-induced aging mice, indicating its great development potential.

Key words: Bama longevity dietary patterns, anti-oxidative stress effect, D-galactose, aging model

中图分类号:

R154.1